Wheat germ agglutinin, labeled with AF647-Cell staining-He Yuan Li Ji

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Wheat germ agglutinin, labeled with AF647

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Product Item Number

Product Specifications

original price

AC15L016

1mg

1580

Download 李记-葛(18616108315)

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Product Description

Wheat germ agglutinin, labeled AF647 (hereinafter referred to as "AF647 WAG"), is a lectin that binds to N-acetyl-D-glucosamine and sialic acid, and may be the brightest WGA conjugate in bright and green fluorescence. It is currently a widely studied and applied type of lectin. Due to the binding of WGA with glycoconjugates, its derivatives and conjugates are widely used for labeling cell membranes and fibrotic scar tissues for fluorescence imaging and analysis. The sugar binding specificity of WGA is related to the sequence of β -1,4-GlcNAc linking residues, i.e. chitinase. Each monomer contains two identical non interacting binding sites that complement 3 or 4 β -1,4-GlcNAc units. Among the detected monosaccharides, only GlcNAc binds to WGA. Mannan does not bind, while GalNAc can only weakly bind. WGA has a high affinity for binding to internal GlcNAc residues in large oligosaccharides containing Gal β (1-4) GlcNAc β (1-3) repeat sequences (i.e., galactosaminoglycans). N-acetylneuraminic acid only participates in low affinity interactions with WGA. WGA displays complex carbohydrate specific patterns that can be used for structural analysis of complex carbohydrates.

Product components

Product Name	Item number	Specifications
Wheat germ agglutinin, labeled with AF647	AC15L016	1mg

Transportation and storage

Blue ice transportation- Store at 20 ℃ away from light and moisture, with a shelf life of 12 months.

This product is only for scientific experimental research and should not be used in clinical diagnosis, treatment, or other fields.

Q：AF647 WGA 用于细胞膜荧光标记时，适合哪些样本类型？
A：可以用于活细胞或固定细胞，广泛用于标记细胞膜和纤维化瘢痕组织，用于荧光成像和分析。小麦胚芽凝集素也可以用于石蜡切片，石蜡切片染色，不建议做透化，透化会让染料进入细胞器出现非特异性染色。

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