Product Description
Heyuan Liji Rapid Cell Cryopreservation Solution (serum-free, protein-free) is suitable for the vast majority of tumor cells and conventional cells, and most stem cells (Heyuan Liji has tested cell types, please follow the official website). Frozen cells can be stored for a long time at -80 ℃ (>6 years). Free from animal derived proteins and serum components, it can effectively reduce contamination by various bacteria, viruses, and mycoplasma, ensuring the safety of frozen cells. This cryopreservation solution contains various cellular nutrients such as DMSO and glucose, which improve cell survival and vitality. It is also suitable for serum-free culture of cells and protein expressing cells.
Product advantages
1. Without the need for programmed cooling, cells can be directly placed in cryopreservation solution and stored in a -80 ℃ refrigerator (-80 ℃ can be stored for more than 6 years), and the cryopreservation solution does not need to be diluted.
2. The cryopreservation solution does not contain serum, greatly reducing the possibility of cell contamination.
3. Do not add serum, do not repeatedly cool down step by step, and do not use liquid nitrogen. Overall, it saves time and money.
Product components
Product Name | Article number | Specifications |
Rapid cell cryopreservation solution (serum-free, protein-free) | AC05L032 | 30mL |
Rapid cell cryopreservation solution (serum-free, protein-free) | AC05L033 | 100mL |
Transportation and Preservation
Transport at room temperature. Store at 4 ℃ and have a shelf life of 24 months.
usage method
冻存液数据库
This product is limited to scientific experimental research and cannot be used in clinical diagnosis, treatment, or other fields.
Fill in the product batch number for querying
Fill in the product batch number for querying
[{"text1":"Traditional cell cryopreservation - stepwise cooling","text2":"Cold storage tube placed at 4 \u2103 -10 minutes \u2192 \u2192 \u2192 -20 \u2103 -30 minutes \u2192 \u2192 \u2192 -80 \u2103 -16 to 18 hours (or overnight) \u2192 \u2192 \u2192 Long term storage in liquid nitrogen tank- 20 \u2103 should not exceed 1 hour to prevent ice crystals from becoming too large and causing massive cell death. This step can also be skipped and directly placed in a -80 \u2103 refrigerator, with a slightly reduced survival rate."},{"text1":"The recovery rate after use is still very good, but I feel that the state of the cells is not as good as using DMSO+serum","text2":"We believe that this state is not related to the cryopreservation solution. The good cell recovery rate indicates a high cell survival rate; Traditional serum cryopreservation is provided with a nutrient, but in fact, cells are in a dormant state during cryopreservation and do not consume any nutrients. There is also sufficient glucose content in our serum-free cell cryopreservation solution. That may be because the cells are already in a state of hunger before cryopreservation, meaning that they have been detached from nutrients for too long during the cryopreservation process. This is also why we require serum-free cryopreservation solutions to be rapidly frozen."},{"text1":"Is Li Ji Biological's rapid cell cryopreservation solution not as viscous as other brands?","text2":"Each company has different formulas, with the viscous ones generally using polypropylene alcohol and the polyethylene glycol used by Li Ji Biotechnology; After testing, our company believes that low viscosity will be more convenient for customers to operate"},{"text1":"Do I still need to put the rapid cell cryopreservation solution in a cryopreservation box?","text2":"The use of rapid cell cryopreservation solution belongs to instantaneous cryopreservation. Do not use a cryopreservation box or add other materials for gradient cryopreservation, because only glucose is used as an energy substance, and gradient cryopreservation can easily damage the cell survival environment"},{"text1":"Previously, rapid freezing solution was used and stored in a -80 \u2103 refrigerator for the first two months, with a particularly good recovery state; After 2 months, the cells cannot recover, why?","text2":"If this situation exists, it can be basically determined that the temperature fluctuation of the refrigerator is relatively large during use. In many laboratories, ultra-low temperature refrigerators are not only used to store cells, but also to store some samples. Team members will constantly turn on and off the refrigerator, causing temperature fluctuations inside the refrigerator. We recommend using liquid nitrogen tanks for long-term storage of cells as a more stable option."}]
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