"Heyuan Liji" is a joint venture between Heyuan Biotechnology (stock code: 688238) and Liji Biotechnology, specializing in the "Liji Biotechnology" and "Life-ilab" reagent brands
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The EZ Trans Lentiviral Packaging Kit from Heyuan Liji contains three components: lentiviral packaging plasmid, control plasmid, and transfection reagent (EZ Trans Regent). The Yuan Li Ji lentivirus packaging kit provides sufficient and high-quality control plasmids and packaging plasmids, eliminating the need for experimental personnel to extract plasmids.
The expression system of HeYuan LiJi lentivirus vector consists of pLenti lentivirus vector and Lentiviral Mix plasmid. The pLenti slow vector contains HIV basic elements 5 'LTR and 3' LTR, as well as other auxiliary elements such as WPRE. The expression frame is U6 shRNA (NC) - CMV-EGFP-2A-Puro, which contains green fluorescence and puro eukaryotic resistance. Customers can modify the plenti vector according to different experimental purposes for promoter, gene function research, stable strain screening, etc. Lentiviral Mix can express various essential components required for virus packaging and is compatible with second-generation and third-generation slow virus packaging systems.
Item number | Specifications | Components | ||
A: Control plasmid | B: Packaging plasmid | C: Transfection reagent | ||
AC04L505 | 5T | 16μg | 110μg | 450μL |
AC04L510 | 10T | 32μg | 220μg | 900μL |
AC04L520 | 20T | 64μg | 330μg | 1800μL |
Blue ice transportation. A. Component B is stored at -20 ℃ and component C is stored at 4 ℃, with a shelf life of 12 months.
This product is only for scientific experimental research and should not be used in clinical diagnosis, treatment, or other fields.
Q:如果不小心将质粒放在4℃了,还能使用吗?
A:李记生物的质粒非常稳定,4℃两周基本没问题;长期保存的话建议放在-20℃或-80℃。
Q:质粒的浓度是多少?还需要去内毒素吗?
A:李记生物提供质粒浓度为1ug/uL,不需要客户再去内毒素了。
Q:如果认为提供的质粒浓度不够,是不是可以自己再转化?使用什么抗性?
A:可以自己再转化,Amp抗性。
Q:EZ Trans慢病毒包装试剂盒可以用来包装干扰表达质粒还是过表达质粒?
A:EZ Trans慢病毒包装试剂盒只能用来包装干扰表达质粒,由 U6 启动子驱动,无真核表达的 ATG 起始、KOZAK 序列、终止子,仅能转录出小 RNA。
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