"Heyuan Liji" is a joint venture between Heyuan Biotechnology (stock code: 688238) and Liji Biotechnology, specializing in the "Liji Biotechnology" and "Life-ilab" reagent brands
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EZ lentivirus infection enhancer (hereinafter referred to as "EZ enhancer") can significantly enhance the efficiency of retroviral infection in cells. Only through formula optimization, this product further enhances the adsorption of sialic acid and virus particles on the cell surface, greatly increasing the efficiency of virus infection in cells. After optimizing the formula, the side effects of the reagent are reduced, the dosage is more stable, and it is more widely applicable to cells.
Significant effect:It can significantly enhance the effectiveness of slow virus infection and effectively save virus usage;
Strong adaptability:almost no cytotoxicity, can adapt to sensitive cells;
Good stability: The product is stable and has a long storage time.
Product Name | Item number | Specifications |
EZ lentivirus infection enhancer | AC04L443 | 250uL |
EZ lentivirus infection enhancer | AC04L444 | 500uL |
Blue ice transportation- Stored at 20 ℃, with a shelf life of 12 months.
This product is only for scientific experimental research and should not be used in clinical diagnosis, treatment, or other fields.
Q:感染贴壁细胞后,需要注意什么?
A:病毒感染细胞72小时后,如载体上有荧光蛋白(GFP、mCherry等元件)可在荧光显微镜下观察细胞,判断慢病毒感染目的细胞的效率。若荧光弱,可延长至96小时后观察。根据后续实验内容,选择合适的时间点进行实验。若载体上不含荧光蛋白,则无法直接通过荧光显微镜判断病毒感染效率,可进行qPCR、WB或目的抗体免疫荧光或目的抗体流式检测病毒表达情况。
Q:感染悬浮细胞后,需要注意什么?
A:病毒感染细胞72小时后,在荧光显微镜下观察细胞,判断慢病毒感染目的细胞的效率。若荧光弱,可延长至96小时后观察。根据后续实验内容,选择合适的时间点进行实验。
Q:感染原代神经元后,需要注意什么?
A:病毒感染细胞72小时后,在荧光显微镜下观察细胞,判断感染的效率。若荧光弱,可延长至96小时后观察。根据后续实验内容,选择合适的时间点进行实验。
Q:李记这款产品对比国产B品牌的优势在哪里?
A:新型实验试剂Polybrene-pus病毒感染增强液是一种阳离子聚合物,通过与细胞膜直接相互作用,提高细胞膜的通透性进而介导病毒颗拉进入细胞中来增加慢病毒对细胞的感染效率,对慢病毒感染的效果明显优于polybrene。本产品可以极大提高细胞感染效率,与Polybrene相比,没有细胞毒性,感染后第二天无需换液。当使用常规培养基感染效果不佳时,可以在培养基lybrene-plus感染液,工作终浓度为1 ug/ul。优点:可以提高病毒的感染效果,所以节省了病毒用量,节约成本。产品稳定,保存时间长。
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